J. Iran. Chem. Soc., Vol. 8, No. 1 March 2011, pp.115~122.

Current location: JICS Archive > Vol. 8 > No. 1 > Articles : 11

Modeling of DNA Hybridization Detection Using Methylene Blue as an Electroactive Label

B. Sehatniaa, b, F. Golabic, M.H. Pournaghi-Azard, R.E. Sabzia and M.S. Hejazic, e,*

aDepartment of Chemistry, Faculty of Science, Urmia University, Urmia, Iran
bArtemia & Aquatic Animals Research Institute, Urmia University, Urmia, Iran
cFaculty of Pharmacy, Tabriz University of  Medical Sciences, Tabriz, Iran
dElectroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
eDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Mathematical modeling of methylene blue (MB) signal in ssDNA and dsDNA on pencil graphite electrode is described. A DNA biosensor was developed based on MB signal. The probe and target DNAs were 20 mer oligonucleotides corresponding to consensus sequence of HPV major capsid protein L1 gene. Hybrids of various complementary and non-complementary oligonucleotides with the probe were considered as dsDNA with different hybridization degrees. Modeling was developed by incorporation of only the stable forms of dsDNA hybrids. Effect of hybridization degree on current signal in various forms was studied. A factor named AHP (Average Hybridization Percentage) for verifying the hybridization events was defined. Results showed that there is a significant mathematical relation between the calculated AHP and MB signals.

Keywords: Biosensor, Pencil graphite electrode, Methylene blue, Modeling, DNA hybridization

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