J. Iran. Chem. Soc., Vol. 7, No. 4 December 2010, pp.840~845.

Current location: JICS Archive > Vol. 7 > No. 4 > Articles : 7

A Novel and Efficient Approach for the Amidation of C-Terminal Peptides

A. Arabanian, M. Mohammadnejad and S. Balalaie*

Peptide Chemistry Research Center, K.N. Toosi University of Technology, P.O. Box 15875-4416, Tehran, Iran

A highly efficient and practical synthesis of C-terminal amidated peptides has been developed. According to this approach, amidation of the C-terminus of peptides was carried out using NH4Cl, alkylammonium chloride (RNH3Cl) and semicarbazide hydrochloride in the presence of TBTU as a coupling reagent and a tertiary amine as the base at room temperature in good to high yields. Some opioid peptides such as enkephalin derivatives were synthesized according to this novel method.

Keywords: Met-enkephalin, Leu-enkephalin, Solid phase peptide synthesis, Amidation, C-Terminal amidated peptides

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