J. Iran. Chem. Soc., Vol. 7, No. 4 December 2010, pp.1004~1011.

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Application of Molecularly Imprinted Polymer for Solid Phase Extraction and Preconcentration of Hydrochlorothiazide in Pharmaceutical and Serum Sample Analysis

B. Rezaei*, S. Mallakpour and O. Rahmanian

Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111 I.R. Iran

A new polymeric sorbent prepared by utilizing molecular imprinting technology was used for the selective extraction of hydrochlorothiazide (HCT) from pharmaceutical and human serum sample. The molecularly imprinted polymer (MIP) was prepared using HCT as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EDMA) as the cross-linker monomer, and dimethylformamide (DMF) as a solvent. The optimized conditions of MIPs as a selective sorbent for the preconcentration of the HCT were studied. The results showed that the drug could be quantitatively and selectively maintained in the column to be then eluted from the sorbent by using methanol-acetic acid mixture (9:1). HCT could be determined spectrophotometrically at λmax = 270 nm. This method made it possible to quantitize HCT in the range of 0.1-21.0 �µg ml-1, by less than 0.55% of RSD%, with a detection limit (S/N = 3) of 0.073 ng ml-1. The preconcentration factor of 1000, recoveries of up to 96% and retention capacity of 75.0 mg g-1 were achieved using this technique.

Keywords: Hydrochlorothiazide, Molecularly imprinted polymer, Solid-phase extraction, Preconcentration

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