J. Iran. Chem. Soc., Vol. 7, No. 1 March 2010, pp.142~149.

Current location: JICS Archive > Vol. 7 > No. 1 > Articles : 15

Application of Aqueous Mixtures of Polypropylene Glycol or Polyethylene Glycol with Salts in Proteomic Analysis

A.R. Salabata,*, M.H. Abnosib and A. Motaharia

aChemistry Department, Arak University, P.O. Box 38156-879, Arak, Iran
bBiology Department, Arak University, P.O. Box 38156-879, Arak, Iran

      Partitioning of Bovine serum albumin (BSA), β-lactoglobulin (β-LG) and zein as model proteins in aqueous two-phase systems (S) containing polypropylene glycol (PPG425) or polyethylene glycol (PEG 6000) and salts (MgSO4, (NH4)2SO4, Na2SO4) is presented in this paper. The effects of different factors such as tie-line length, salt type and polymer type on the partition coefficient and recovery percent of proteins were analysed. The model proteins were separated by these systems (S) and directly used for gel electrophoresis without separating the target proteins from phase-forming reagents. The results revealed that the S, studied in this work could be used as a novel prefractionation method in proteomic analysis and could separate proteomic proteins in multigroup by one step extraction.

Keywords: Proteomic, Aqueous two-phase system (S), Polypropylene glycol, Partitioning, Protein prefractionation

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