J. Iran. Chem. Soc., Vol. 6, No. 1 March 2009, pp.145~152.

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DNA-Binding and Cytotoxicity of the Cobalt(III) Ethylenediamine Pyrazole Complex [Co(en)2(pyz)2]3+

P. Nagababua, J. Naveena lavanya lathaa, M. Rajeshb and S. Satyanarayanaa,*

aDepartment of Chemistry, Osmania University
bDepartment of Biochemistry, Osmania University, Hyderabad, Andhra Pradesh, India

The complex of cobalt(III) ethylenediamine was synthesized, isolated and characterized by UV-Vis, IR, and 1H NMR spectral methods. The binding of the complex with calf thymus DNA was investigated by absorption and emission spectroscopy, viscosity measurements, DNA melting and DNA photocleavage. The spectroscopic studies together with the viscosity measurements and DNA melting studies indicated that the complex binds to calf thymus DNA in a nonintercalative mode. This complex is found to promote photocleavage of the DNA plasmid pBR322 and shows a cytotoxic effect against CHO cells.

Keywords: DNA-binding, Cobalt(III) complexes, Bioinorganic chemistry, Cytotoxic activity

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