Current location: JICS Archive > Vol. 5 > No. 3 > Articles : 13

Determination of Furazolidone in Urine by Square-Wave Voltammetric Method

H. Parham* and B. Aibaghi Esfahani

Department of Chemistry, Faculty of Sciences, Shahid Chamran University, Ahvaz, Iran

A fast, simple and sensitive square-wave voltammetric (SWV) method for the determination of trace amounts of furazolidone (FZ) in urine is reported. A three-electrode system containing stationary mercury dropping (SMDE) working electrode, Pt auxiliary electrode and Ag/AgCl reference electrode was used throughout. Briton-Rabinson buffer solution is used as both pH adjusting agent and supporting electrolyte. The calibration graph showed good linearity in the concentration range of 20-900 ng ml^-1 of furazolidone with a regression coefficient of 0.9996. The equation Δ"(i) = 0.0095CFZ + 0.234 was used for calculation of furazolidone concentration in the sample solution, where CFZ is the concentration of furazolidone in ng ml^-1 and Δ"(i) is the difference between voltammogram peak currents of sample and blank solution. The RSD for 8 replicate measurements of a 60 ng ml^-1 solution and LOD of the proposed method were found to be 2.2% and 5.2 ng ml^-1, respectively. The procedure was successfully applied to the determination of furazolidone in urine samples.

Keywords: Furazolidone, SW Voltammetry, Urine, Determination